{"id":31,"date":"2021-10-28T08:15:03","date_gmt":"2021-10-28T08:15:03","guid":{"rendered":"http:\/\/www.celetrix.cn\/celetrix\/?page_id=31"},"modified":"2023-08-02T06:19:17","modified_gmt":"2023-08-02T06:19:17","slug":"genome-editing","status":"publish","type":"page","link":"https:\/\/www.celetrix.cn\/zh\/genome-editing\/","title":{"rendered":"\u57fa\u56e0\u7f16\u8f91"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"31\" class=\"elementor elementor-31\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2cd4bcc0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2cd4bcc0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-42465f82\" 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data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Celetrix\u7535\u8f6c\u4eea\u5728CRISPR\/Cas9\u57fa\u56e0\u7f16\u8f91\u9886\u57df\u7684\u5e94\u7528\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6bf01427 elementor-widget elementor-widget-text-editor\" data-id=\"6bf01427\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u4f7f\u7528Celetrix\u7535\u8f6c\u65b9\u6cd5\uff0c\u6211\u4eec\u53ef\u4ee5\u5c06RNP(Cas9\u86cb\u767d\u548cgRNA\u590d\u5408\u7269)\u6216\u8005Cas9\u8d28\u7c92\u5bfc\u5165\u7ec6\u80de\uff0c\u8fdb\u884c\u9ad8\u6548\u57fa\u56e0\u7f16\u8f91\u3002<\/p><div><p>RNP\u7535\u8f6c\u5bf9\u4e8e\u7ec6\u80de\u7684\u6bd2\u6027\u5f88\u5c0f\uff0c\u57fa\u56e0\u6572\u9664\u6548\u7387\u4e00\u822c\u53ef\u4ee5\u8fbe\u523090%\u4ee5\u4e0a\uff0c\u53ef\u4ee5\u9002\u7528\u4e8e\u51e0\u4e4e\u6240\u6709\u7684\u7ec6\u80de\u79cd\u7c7b\u3002\u5bf9\u4e8eT\u7ec6\u80de\u3001NK\u7ec6\u80de\u7b49\u539f\u4ee3\u7ec6\u80de\u548c\u5404\u79cd\u5e72\u7ec6\u80de\uff0cCas9\u8d28\u7c92\u7535\u8f6c\u6709\u8f83\u5927\u6bd2\u6027\uff0c\u6211\u4eec\u63a8\u8350\u4f7f\u7528RNP\u8fdb\u884c\u57fa\u56e0\u7f16\u8f91\u3002<\/p><p>\u539f\u4ee3T\u7ec6\u80de\u4e0d\u7ba1\u662f\u672a\u523a\u6fc0\u7684PBMC\u6216\u8005\u523a\u6fc0\u57f9\u517b\u7684T\u7ec6\u80de\u90fd\u53ef\u4ee5\u4f7f\u7528RNP\u8fdb\u884c\u9ad8\u6548\u57fa\u56e0\u7f16\u8f91\uff0c\u53ea\u8981\u8c03\u8282\u5404\u81ea\u6240\u9700\u7684\u7535\u538b\u5c31\u53ef\u4ee5\u3002PBMC\u76f4\u63a5\u505a\u57fa\u56e0\u7f16\u8f91\uff0c\u9700\u8981\u4f7f\u7528PBMC\u6a21\u5f0f\uff0c\u4f7f\u7528\u8f83\u9ad8\u7684\u7535\u538b\u3002\u800c\u523a\u6fc02-3\u5929\u7684T\u7ec6\u80de\u5728\u7ec6\u80de\u7cfb\u6a21\u5f0f\u4e0b\u7528\u8f83\u4f4e\u7535\u538b\u5c31\u53ef\u4ee5\u7535\u8f6cRNP\u3002\u6bd4\u5982TCR\u6572\u9664\u7684\u5b9e\u9a8c\uff0c\u6211\u4eec\u4f7f\u75282-3\u5929\u523a\u6fc0\u57f9\u517b\u7684T\u7ec6\u80de\uff0c\u53ef\u4ee5\u8fbe\u523092%\u7684CD3\u6572\u9664\u6548\u7387\uff0c\u7ec6\u80de\u751f\u957f\u72b6\u6001\u548c\u672a\u7535\u8f6c\u7684\u5bf9\u7167\u7ec6\u80de\u76f8\u4f3c\u3002<\/p><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d90d8f8 elementor-widget elementor-widget-image\" data-id=\"d90d8f8\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/12\/\u5fae\u4fe1\u56fe\u7247_20211224173836.png\" title=\"\u5fae\u4fe1\u56fe\u7247_20211224173836\" alt=\"\u5fae\u4fe1\u56fe\u7247_20211224173836\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8aa837c elementor-widget elementor-widget-text-editor\" data-id=\"8aa837c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div><div><p><span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\">T\u7ec6\u80de\u3001NK\u7ec6\u80de\u548c\u9020\u8840\u5e72\u7ec6\u80de\u7684\u57fa\u56e0\u7f16\u8f91\u5728\u57fa\u56e0\u6cbb\u7597\u9886\u57df\u6bd4\u8f83\u91cd\u8981\uff0c\u6211\u4eec\u6709\u6210\u719f\u7684\u65b9\u6848\u53ef\u4ee5\u5e2e\u52a9\u60a8\u5feb\u901f\u5b8c\u6210\u5b9e\u9a8c\u3002\u53e6\u5916\uff0c\u6211\u4eec\u4e5f\u6709\u5355\u6b21\u7535\u8f6c10ml\u7684\u5927\u4f53\u79ef\u7535\u8f6c\u4eea\u53ef\u4ee5\u5355\u6b21\u5904\u740610E9\u91cf\u7ea7\u7684\u7ec6\u80de\u3002<\/span><\/p><p>\u5bf9\u4e8eiPSC\u7ec6\u80de\uff0c\u7535\u8f6cRNP\u4e5f\u662f\u4f7f\u7528\u7ec6\u80de\u7cfb\u6a21\u5f0f\uff0c\u7b80\u5355\u8c03\u8282\u7535\u538b\u5c31\u53ef\u4ee5\u8fbe\u5230\u9ad8\u6548\u7387\u3002\u6bd4\u5982\u5728AAVS1\u548cOCT4\u57fa\u56e0\u4f4d\u70b9\u4e0a\uff0c\u4f7f\u7528RNP\u7535\u8f6c\u53ef\u4ee5\u8fbe\u5230\u8f83\u9ad8\u7684\u6572\u9664\u6548\u7387\u3002\u5982\u679c\u9700\u8981\u8fdb\u4e00\u6b65\u63d0\u9ad8\u6572\u9664\u6548\u7387\uff0c\u53ef\u4ee5\u4f7f\u7528\u8f83\u9ad8\u6d53\u5ea6\u7684RNP\uff0c\u6bd4\u5982\u572820ul\u7535\u51fb\u7ba1\u91cc\u9762\u4f7f\u752810ug\u7684Cas9\u86cb\u767d\u548c5ug\u7684gRNA<\/p><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div 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class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div><div>\u4f7f\u7528\u8d28\u7c92\u7535\u8f6c\u8fdb\u884c\u57fa\u56e0\u7f16\u8f91\uff0c\u9002\u5408\u4e8e\u666e\u901a\u7ec6\u80de\u7cfb\u3002\u5e26Cas9\u57fa\u56e0\u548cgRNA\u7684\u8d28\u7c92\u4e00\u822c\u5206\u5b50\u91cf\u6bd4\u8f83\u5927\uff0c\u7535\u8f6c\u65f6\u5177\u6709\u4e00\u5b9a\u7684\u6bd2\u6027\uff0c\u4f46\u662f\u7ec6\u80de\u7cfb\u751f\u957f\u901f\u5ea6\u5feb\u53ef\u4ee5\u627f\u53d7\u3002\u7531\u4e8e\u8d28\u7c92\u8fdb\u884c\u57fa\u56e0\u7f16\u8f91\u6548\u7387\u6bd4RNP\u4f4e\uff0c\u4e00\u822c\u9700\u8981\u8fdb\u884c\u52a0\u836f\u7b5b\u9009\uff0c\u63d0\u9ad8\u57fa\u56e0\u7f16\u8f91\u7684\u6548\u7387\u3002\u52a0\u836f\u7b5b\u9009\u6709\u4e24\u79cd\u65b9\u6848\uff0c\u4e00\u79cd\u662f\u5e38\u89c4\u7684\u957f\u65f6\u95f4\u7b5b\u9009\uff0c\u5f97\u5230\u7684\u7ec6\u80de\u4f1a\u7a33\u5b9a\u8868\u8fbeCas9\u86cb\u767d\u548cgRNA\uff0c\u540c\u65f6\u5e26\u6709\u7f16\u8f91\u7684\u57fa\u56e0\u3002THP-1\u7ec6\u80de\u4f7f\u7528\u6211\u4eec\u7684\u8f85\u52a9\u8bd5\u5242\u7535\u8f6cCas9-gRNA-GFP-Puro\u8d28\u7c92\u540e\uff0c\u7ecf\u8fc7\u957f\u671f\u7b5b\u9009\u53ef\u4ee5\u5f97\u5230\u5168\u90e8GFP\u9633\u6027\u7684\u7ec6\u80de\uff0c\u8fd9\u4e9b\u7ec6\u80de\u7684\u57fa\u56e0\u7f16\u8f91\u4e5f\u5df2\u7ecf\u5927\u90e8\u5206\u5b8c\u6210 \u3002<\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-108ceac elementor-widget elementor-widget-image\" data-id=\"108ceac\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"331\" height=\"156\" src=\"https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/12\/\u56fe\u72474.png\" class=\"attachment-large size-large wp-image-993\"  srcset=\"https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/12\/\u56fe\u72474.png 331w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/12\/\u56fe\u72474-300x141.png 300w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/12\/\u56fe\u72474-18x8.png 18w\" sizes=\"(max-width: 331px) 100vw, 331px\" title=\"4.png\" alt=\"4.png\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-071bb32 elementor-widget elementor-widget-text-editor\" data-id=\"071bb32\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u4f7f\u7528\u8d28\u7c92\u7535\u8f6c\u7684\u65b9\u6cd5\u505a\u57fa\u56e0\u7f16\u8f91\u4e00\u822c\u9700\u8981\u65f6\u95f4\u6bd4\u8f83\u957f\u3002\u7531\u4e8eCeletrix\u7535\u8f6c\u6548\u7387\u9ad8\u5e76\u4e14\u7ec6\u80de\u5b58\u6d3b\u7387\u9ad8\uff0c\u6211\u4eec\u6709\u53e6\u4e00\u79cd\u7279\u6b8a\u7684\u5feb\u901f\u7b5b\u9009\u65b9\u6cd5\u5728\u8d28\u7c92\u77ac\u8f6c\u9636\u6bb5\u5b8c\u6210\u9ad8\u6548\u57fa\u56e0\u7f16\u8f91\u3002\u5f97\u5230\u7684\u7ec6\u80de\u5e26\u6709\u9ad8\u6548\u57fa\u56e0\u7f16\u8f91\uff0c\u5e76\u4e14\u4e0d\u5e26\u8d28\u7c92\u4e0a\u7684gRNA\u548c\u7b5b\u9009\u6807\u8bb0\uff0c\u65b9\u4fbf\u8fdb\u884c\u53e6\u4e00\u4e2a\u57fa\u56e0\u7684\u7f16\u8f91\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-107fb73e\" data-id=\"107fb73e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-21740019 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"21740019\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot; aria-hidden=\\&quot;true\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-21740019\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-287\"><a href=\"https:\/\/www.celetrix.cn\/zh\/genome-editing\/\" class=\"elementor-item\">\u57fa\u56e0\u7f16\u8f91<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-285\"><a href=\"https:\/\/www.celetrix.cn\/zh\/antibody-production\/\" class=\"elementor-item\">\u6297\u4f53\u751f\u4ea7<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-288\"><a href=\"https:\/\/www.celetrix.cn\/zh\/stem-cell-therapy\/\" class=\"elementor-item\">\u5e72\u7ec6\u80de\u7535\u8f6c<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-286\"><a href=\"https:\/\/www.celetrix.cn\/zh\/car-tcar-nk\/\" class=\"elementor-item\">CAR-T\u548cCAR-NK<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-21740019\" class=\"elementor-nav-menu sm-vertical\"><li 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tabindex=\"-1\">CAR-T\u548cCAR-NK<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>\u5168\u65b0\u9ad8\u6548\u7ec6\u80de\u7535\u8f6c\u4eea \u9875\u9762 Celetrix\u7535\u8f6c\u4eea\u5728CRISPR\/Cas9\u57fa\u56e0\u7f16\u8f91\u9886\u57df\u7684\u5e94\u7528 \u4f7f\u7528Celetrix\u7535\u8f6c\u65b9\u6cd5\uff0c\u6211\u4eec\u53ef\u4ee5\u5c06RNP(Cas9\u86cb\u767d\u548cgRNA\u590d\u5408\u7269)\u6216\u8005Cas9\u8d28\u7c92\u5bfc\u5165\u7ec6\u80de\uff0c\u8fdb\u884c\u9ad8\u6548\u57fa\u56e0\u7f16\u8f91\u3002 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