{"id":37,"date":"2021-10-28T08:15:40","date_gmt":"2021-10-28T08:15:40","guid":{"rendered":"http:\/\/www.celetrix.cn\/celetrix\/?page_id=37"},"modified":"2023-08-02T06:14:20","modified_gmt":"2023-08-02T06:14:20","slug":"electroporation-mechanism","status":"publish","type":"page","link":"https:\/\/www.celetrix.cn\/zh\/electroporation-mechanism\/","title":{"rendered":"\u7535\u8f6c\u57fa\u672c\u539f\u7406"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"37\" class=\"elementor elementor-37\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3e7b720a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3e7b720a\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-6701cade\" data-id=\"6701cade\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7cd48319 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"7cd48319\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.cn\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>\u5168\u65b0\u9ad8\u6548\u7ec6\u80de\u7535\u8f6c\u4eea<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-37cd805 elementor-widget elementor-widget-heading\" data-id=\"37cd805\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u7535\u8f6c\u57fa\u672c\u539f\u7406<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-abe5a46 elementor-widget elementor-widget-text-editor\" data-id=\"abe5a46\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>1. \u7535\u8f6c\u6280\u672f\u539f\u7406<\/strong><\/p><p style=\"margin-right: 0px; margin-bottom: 0px; margin-left: 0px; padding: 0px; border: 0px; outline: none; font-family: 'Open Sans', sans-serif; font-size: 13px;\">\u7ec6\u80de\u7535\u8f6c\u67d3\uff08\u7535\u8f6c\uff09\uff0c\u4e5f\u53eb\u7ec6\u80de\u7535\u7a7f\u5b54 (electroporation)\uff0c\u662f\u628a\u5916\u6e90\u5927\u5206\u5b50\u7269\u8d28DNA\u3001RNA\u3001siRNA\u3001\u86cb\u767d\u8d28\u7b49\u4ee5\u53ca\u4e00\u4e9b\u5c0f\u5206\u5b50\u5bfc\u5165\u7ec6\u80de\u819c\u5185\u90e8\u7684\u91cd\u8981\u65b9\u6cd5\u3002<\/p><p style=\"margin-right: 0px; margin-bottom: 0px; margin-left: 0px; padding: 0px; border: 0px; outline: none; font-family: 'Open Sans', sans-serif; font-size: 13px;\">\u5728\u77ac\u95f4\u5f3a\u5927\u7535\u573a\u7684\u4f5c\u7528\u4e0b\uff0c\u6eb6\u6db2\u4e2d\u7ec6\u80de\u7684\u7ec6\u80de\u819c\u5177\u6709\u4e86\u4e00\u5b9a\u7684\u901a\u900f\u6027\uff0c\u5e26\u7535\u7684\u5916\u6e90\u7269\u8d28\u4ee5\u7c7b\u4f3c\u7535\u6cf3\u7684\u65b9\u5f0f\u8fdb\u5165\u7ec6\u80de\u819c\u3002\u7531\u4e8e\u7ec6\u80de\u819c\u78f7\u8102\u53cc\u5206\u5b50\u5c42\u7684\u7535\u963b\u5f88\u5927\uff0c\u7ec6\u80de\u5916\u90e8\u7535\u6d41\u573a\u4ea7\u751f\u7684\u7ec6\u80de\u4e24\u6781\u7535\u538b\u90fd\u88ab\u7ec6\u80de\u819c\u627f\u53d7\uff0c\u7ec6\u80de\u8d28\u5185\u5206\u5230\u7684\u7535\u538b\u53ef\u4ee5\u5ffd\u7565\u4e0d\u8ba1\uff0c\u7ec6\u80de\u8d28\u5185\u90e8\u51e0\u4e4e\u6ca1\u6709\u7535\u6d41\uff0c\u56e0\u6b64\u4e5f\u51b3\u5b9a\u4e86\u6b63\u5e38\u8303\u56f4\u5185\u7684\u7535\u8f6c\u8fc7\u7a0b\u4e2d\u7ec6\u80de\u6bd2\u6027\u5f88\u5c0f\u3002\u7535\u573a\u4f7fDNA\u7b49\u7269\u8d28\u8fdb\u5165\u7ec6\u80de\u819c\u540e\u53ea\u80fd\u505c\u6b62\u5728\u7ec6\u80de\u819c\u9644\u8fd1\uff0c\u968f\u540e\u7ec6\u80de\u672c\u8eab\u7684\u673a\u5236\u53ef\u4ee5\u5141\u8bb8\u8fd9\u4e9b\u7269\u8d28\u5230\u7ec6\u80de\u6838\u7b49\u5904\u3002<\/p><p style=\"margin-right: 0px; margin-bottom: 0px; margin-left: 0px; padding: 0px; border: 0px; font-family: 'Open Sans', sans-serif; font-size: 13px; outline: none !important;\">\u666e\u901a\u7535\u8f6c\u80fd\u91cf\u8fc7\u9ad8\u53ef\u5bfc\u81f4\u90e8\u5206\u7ec6\u80de\u56e0\u819c\u7834\u574f\u800c\u6b7b\u4ea1\uff0c\u5176\u4e2d\u80fd\u5b58\u6d3b\u4e0b\u6765\u7684\u7ec6\u80de\u5185\u90e8\u90fd\u4e0d\u4f1a\u53d7\u5230\u7535\u6d41\u5f71\u54cd\uff0c\u56e0\u4e3a\u7ec6\u80de\u819c\u5b8c\u6574\u662f\u7ec6\u80de\u5b58\u6d3b\u7684\u5fc5\u8981\u6761\u4ef6\u3002<\/p><p style=\"margin-right: 0px; margin-bottom: 0px; margin-left: 0px; padding: 0px; border: 0px; outline: none; font-family: 'Open Sans', sans-serif; font-size: 13px;\">\u7531\u4e8e\u7535\u8f6c\u6280\u672f\u4f9d\u9760\u7684\u662f\u7269\u7406\u65b9\u6cd5\uff0c\u7ec6\u80de\u8868\u9762\u7684\u5206\u5b50\u7279\u6027\u5bf9\u7535\u8f6c\u5f71\u54cd\u6bd4\u8f83\u5c0f\u3002\u76f8\u6bd4\u5316\u5b66\u8f6c\u67d3\u65b9\u6cd5\u548c\u75c5\u6bd2\u8f7d\u4f53\u8f6c\u67d3\u65b9\u6cd5\uff0c\u7535\u8f6c\u53ef\u4ee5\u7528\u5728\u6240\u6709\u7684\u7ec6\u80de\u79cd\u7c7b\u4e0a\uff0c\u800c\u4e14\u5bb9\u6613\u5b9a\u91cf\u63a7\u5236\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-a0f474e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"a0f474e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-554fea2\" data-id=\"554fea2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1b4cecb elementor-widget elementor-widget-text-editor\" data-id=\"1b4cecb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>2. \u7ec6\u80de\u7535\u8f6c\u7535\u573a\u56fe<\/strong><\/p><div class=\"container\"><div class=\"col-sm-12 col-md-5-5 pc-mr12\"><img decoding=\"async\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\"  width=\"9\" height=\"8\" title=\"20180326165323_118.jpg\" alt=\"20180326165323_118.jpg\" \/>\u00a0\u7ec6\u80de\u819c\u8fd1\u4f3c\u7edd\u7f18\u4f53\uff0c\u7ec6\u80de\u9644\u8fd1\u7535\u8f6c\u6db2\u4e2d\u7535\u6d41\u626d\u66f2\u3002<br \/><img decoding=\"async\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\"  width=\"9\" height=\"8\" title=\"20180326165323_118.jpg\" alt=\"20180326165323_118.jpg\" \/>\u00a0\u5728\u4e32\u8054\u7535\u8def\u4e2d\u7535\u963b\u8d8a\u5927\u5206\u5230\u7535\u538b\u8d8a\u5927\uff0c\u7ec6\u80de\u4e24\u7aef\u7535\u538b\u57fa\u672c\u90fd\u843d\u5728\u4e24\u7aef\u7ec6\u80de\u819c\u4e0a\u3002<br \/><img decoding=\"async\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\"  width=\"9\" height=\"8\" title=\"20180326165323_118.jpg\" alt=\"20180326165323_118.jpg\" \/>\u00a0\u53ea\u6709\u4e00\u4e2a\u7ec6\u80de\u7aef\u9762\u7535\u8f6c\u6709\u6548\u3002<br \/><img decoding=\"async\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\"  width=\"9\" height=\"8\" title=\"20180326165323_118.jpg\" alt=\"20180326165323_118.jpg\" \/>\u00a0\u7ec6\u80de\u8d28\u5185\u5206\u5230\u7535\u538b\u5f88\u5c0f\uff0cDNA\u7535\u8f6c\u8fdb\u5165\u7ec6\u80de\u819c\u540e\u9a6c\u4e0a\u505c\u6b62\uff0c\u540e\u7eed\u9760\u7ec6\u80de\u672c\u8eab\u673a\u5236\u6162\u901f\u6269\u6563\u3002<br \/><img decoding=\"async\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\"  width=\"9\" height=\"8\" title=\"20180326165323_118.jpg\" alt=\"20180326165323_118.jpg\" \/>\u00a0\u7ec6\u80de\u6838\u4e24\u7aef\u7535\u538b\u53ca\u5176\u5fae\u5c0f\uff0c\u5fae\u5c0f\u7684\u7535\u538b\u53c8\u843d\u5728\u6838\u819c\u4e0a\uff0c\u6838\u5185\u90e8\u5b8c\u5168\u6ca1\u6709\u7535\u538b\uff0c\u7535\u8f6c\u6ca1\u6709\u57fa\u56e0\u6bd2\u6027\u3002<\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-55906f4\" data-id=\"55906f4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-490d86f elementor-widget elementor-widget-text-editor\" data-id=\"490d86f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-241 aligncenter\" src=\"http:\/\/celetrix.hy-yun.com\/wp-content\/uploads\/2021\/11\/20180328104530_335.jpg\"  width=\"269\" height=\"280\" srcset=\"https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180328104530_335.jpg 864w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180328104530_335-289x300.jpg 289w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180328104530_335-768x797.jpg 768w\" sizes=\"(max-width: 269px) 100vw, 269px\" title=\"20180328104530_335.jpg\" alt=\"20180328104530_335.jpg\" \/><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-ef2b34d elementor-widget elementor-widget-text-editor\" data-id=\"ef2b34d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>3. \u7535\u8f6c\u6280\u672f\u5e94\u7528<\/strong><\/p><p><img decoding=\"async\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\"  width=\"9\" height=\"8\" title=\"20180326165323_118.jpg\" alt=\"20180326165323_118.jpg\" \/>\u6709\u6548\u7684\u7ec6\u80de\uff1a\u51e0\u4e4e\u6240\u6709\u7684\u771f\u6838\u7ec6\u80de\uff0c\u5305\u62ec\u6240\u6709\u7ec6\u80de\u7cfb\uff0c\u539f\u4ee3\u7ec6\u80de\uff0c\u5e72\u7ec6\u80de\uff0c\u8840\u6db2\u548c\u514d\u75ab\u7c7b\u7ec6\u80de\uff0c\u795e\u7ecf\u7ec6\u80de\uff0c\u690d\u7269\u7ec6\u80de\u548c\u80da\u80ce\u3002<\/p><p><img decoding=\"async\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\"  width=\"9\" height=\"8\" title=\"20180326165323_118.jpg\" alt=\"20180326165323_118.jpg\" \/>\u53ef\u4ee5\u7535\u8f6c\u7684\u7269\u8d28\u5305\u62ecDNA\u3001RNA\u3001siRNA\u3001\u86cb\u767d\u8d28\u7b49\u3002<\/p><p><img decoding=\"async\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\"  width=\"9\" height=\"8\" title=\"20180326165323_118.jpg\" alt=\"20180326165323_118.jpg\" \/>\u9002\u7528\u5b9e\u9a8c\uff1a\u77ac\u65f6\u6216\u7a33\u5b9a\u8868\u8fbe\u86cb\u767d\u8d28\u3001\u57fa\u56e0\u6572\u9664\u4e0e\u6572\u5165\u3001siRNA\u548c\u5176\u4ed6\u5206\u5b50\u673a\u5236\u7814\u7a76\u3002<\/p><p><img decoding=\"async\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\"  width=\"9\" height=\"8\" title=\"20180326165323_118.jpg\" alt=\"20180326165323_118.jpg\" \/>\u79d1\u7814\u9886\u57df\uff1a\u7ec6\u80de\u5206\u5b50\u751f\u7269\u5b66\u3001\u514d\u75ab\u5b66\u3001\u8840\u6db2\u5b66\u3001\u795e\u7ecf\u5b66\u3001\u764c\u75c7\u7814\u7a76\u3001\u65b0\u836f\u5f00\u53d1\u7b49\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fed322c elementor-widget elementor-widget-text-editor\" data-id=\"fed322c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>4. \u4e3a\u4ec0\u4e48\u7535\u8f6c\u76f4\u63a5\u8fdb\u5165\u7ec6\u80de\u6838\u662f\u9519\u8bef\u7684<\/strong><\/p><div>1.\u7ec6\u80de\u6838\u8f6c\u67d3\u8fdd\u53cd\u7269\u7406\u5b66\u539f\u7406\u3002<\/div><p>\u7ec6\u80de\u819c\u8fd1\u4f3c\u7edd\u7f18\u4f53\uff0c\u7ec6\u80de\u9644\u8fd1\u7535\u8f6c\u6db2\u4e2d\u7535\u6d41\u626d\u66f2\u3002\u5728\u4e32\u8054\u7535\u8def\u4e2d\u7535\u963b\u8d8a\u5927\u5206\u5230\u7535\u538b\u8d8a\u5927\uff0c\u7ec6\u80de\u4e24\u7aef\u7535\u538b\u57fa\u672c\u90fd\u843d\u5728\u4e24\u7aef\u7ec6\u80de\u819c\u4e0a\u3002\u53ea\u6709\u4e00\u4e2a\u7ec6\u80de\u7aef\u9762\u7535\u8f6c\u6709\u6548\u3002\u7ec6\u80de\u8d28\u5185\u5206\u5230\u7535\u538b\u5f88\u5c0f\uff0cDNA\u7535\u8f6c\u8fdb\u5165\u7ec6\u80de\u819c\u540e\u9a6c\u4e0a\u505c\u6b62\uff0c\u540e\u7eed\u9760\u7ec6\u80de\u672c\u8eab\u673a\u5236\u6162\u901f\u6269\u6563\u3002\u7ec6\u80de\u6838\u4e24\u7aef\u7535\u538b\u53ca\u5176\u5fae\u5c0f\uff0c\u5fae\u5c0f\u7684\u7535\u538b\u53c8\u843d\u5728\u6838\u819c\u4e0a\uff0c\u6838\u5185\u90e8\u5b8c\u5168\u6ca1\u6709\u7535\u538b\uff0c\u7535\u8f6c\u6ca1\u6709\u57fa\u56e0\u6bd2\u6027\u3002<\/p><p><img decoding=\"async\" class=\"alignnone size-full wp-image-525\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095628_133.png\"  width=\"536\" height=\"272\" srcset=\"https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095628_133.png 536w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095628_133-300x152.png 300w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095628_133-18x9.png 18w\" sizes=\"(max-width: 536px) 100vw, 536px\" title=\"20210610095628_133.png\" alt=\"20210610095628_133.png\" \/><\/p><p>2.\u6587\u732e\u4e2d\u5df2\u7ecf\u62a5\u5bfc\u7535\u8f6c\u540eDNA\u4ec5\u5728\u7ec6\u80de\u819c\u9644\u8fd1\uff08Golzio et al.\uff09\u3002<\/p><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-526\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095647_972.png\"  width=\"503\" height=\"431\" srcset=\"https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095647_972.png 503w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095647_972-300x257.png 300w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095647_972-14x12.png 14w\" sizes=\"(max-width: 503px) 100vw, 503px\" title=\"20210610095647_972.png\" alt=\"20210610095647_972.png\" \/><\/p><p>3.\u7ec6\u80de\u6838\u8f6c\u67d3\u6ca1\u6709\u5b9e\u9a8c\u8bc1\u636e\u3002<\/p><p>4.\u7ec6\u80de\u672c\u8eab\u53ef\u4ee5\u8ba9DNA\u8fdb\u5165\u7ec6\u80de\u6838\u3002\u6bd4\u5982Celetrix\u7535\u8f6c\u540e30\u5206\u949f\u5c31\u53ef\u4ee5\u770b\u5230GFP\u8367\u5149\u8868\u8fbe\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-7f5be76a\" data-id=\"7f5be76a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5c1c01c4 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"5c1c01c4\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot; aria-hidden=\\&quot;true\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-5c1c01c4\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-99\"><a href=\"https:\/\/www.celetrix.cn\/zh\/electroporation-mechanism\/\" class=\"elementor-item\">\u7535\u8f6c\u57fa\u672c\u539f\u7406<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-100\"><a href=\"https:\/\/www.celetrix.cn\/zh\/nucleus-transfection\/\" class=\"elementor-item\">\u8f6c\u67d3\u7ec6\u80de\u6838\u9519\u8bef\u6982\u5ff5<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-98\"><a href=\"https:\/\/www.celetrix.cn\/zh\/celetrix-advantage\/\" class=\"elementor-item\">Celetrix\u7ec6\u80de\u7535\u8f6c\u4f18\u52bf<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-103\"><a href=\"https:\/\/www.celetrix.cn\/zh\/vs-cuvette\/\" class=\"elementor-item\">\u4e0e\u7535\u6781\u676f\u6bd4\u8f83<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-102\"><a href=\"https:\/\/www.celetrix.cn\/zh\/vs-capillary\/\" class=\"elementor-item\">\u4e0e\u6bdb\u7ec6\u7ba1\u6bd4\u8f83<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-101\"><a href=\"https:\/\/www.celetrix.cn\/zh\/rnp-mrna-sirna-vs-plasmid\/\" class=\"elementor-item\">mRNA\/ siRNA\u7535\u8f6c<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-5c1c01c4\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-99\"><a href=\"https:\/\/www.celetrix.cn\/zh\/electroporation-mechanism\/\" class=\"elementor-item\" tabindex=\"-1\">\u7535\u8f6c\u57fa\u672c\u539f\u7406<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-100\"><a href=\"https:\/\/www.celetrix.cn\/zh\/nucleus-transfection\/\" class=\"elementor-item\" tabindex=\"-1\">\u8f6c\u67d3\u7ec6\u80de\u6838\u9519\u8bef\u6982\u5ff5<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-98\"><a href=\"https:\/\/www.celetrix.cn\/zh\/celetrix-advantage\/\" class=\"elementor-item\" tabindex=\"-1\">Celetrix\u7ec6\u80de\u7535\u8f6c\u4f18\u52bf<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-103\"><a href=\"https:\/\/www.celetrix.cn\/zh\/vs-cuvette\/\" class=\"elementor-item\" tabindex=\"-1\">\u4e0e\u7535\u6781\u676f\u6bd4\u8f83<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-102\"><a href=\"https:\/\/www.celetrix.cn\/zh\/vs-capillary\/\" class=\"elementor-item\" tabindex=\"-1\">\u4e0e\u6bdb\u7ec6\u7ba1\u6bd4\u8f83<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-101\"><a href=\"https:\/\/www.celetrix.cn\/zh\/rnp-mrna-sirna-vs-plasmid\/\" class=\"elementor-item\" tabindex=\"-1\">mRNA\/ siRNA\u7535\u8f6c<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>\u5168\u65b0\u9ad8\u6548\u7ec6\u80de\u7535\u8f6c\u4eea \u9875\u9762 \u7535\u8f6c\u57fa\u672c\u539f\u7406 1. \u7535\u8f6c\u6280\u672f\u539f\u7406 \u7ec6\u80de\u7535\u8f6c\u67d3\uff08\u7535\u8f6c\uff09\uff0c\u4e5f\u53eb\u7ec6\u80de\u7535\u7a7f\u5b54 (electroporation)\uff0c\u662f\u628a\u5916\u6e90\u5927\u5206\u5b50\u7269\u8d28DNA\u3001RNA\u3001siRNA\u3001\u86cb\u767d\u8d28\u7b49\u4ee5\u53ca\u4e00\u4e9b\u5c0f\u5206\u5b50\u5bfc\u5165\u7ec6\u80de\u819c\u5185\u90e8\u7684\u91cd\u8981\u65b9\u6cd5\u3002 \u5728\u77ac\u95f4\u5f3a\u5927\u7535\u573a\u7684\u4f5c\u7528\u4e0b\uff0c\u6eb6\u6db2\u4e2d\u7ec6\u80de\u7684\u7ec6\u80de\u819c\u5177\u6709\u4e86\u4e00\u5b9a\u7684\u901a\u900f\u6027\uff0c\u5e26\u7535\u7684\u5916\u6e90\u7269\u8d28\u4ee5\u7c7b\u4f3c\u7535\u6cf3\u7684\u65b9\u5f0f\u8fdb\u5165\u7ec6\u80de\u819c\u3002\u7531\u4e8e\u7ec6\u80de\u819c\u78f7\u8102\u53cc\u5206\u5b50\u5c42\u7684\u7535\u963b\u5f88\u5927\uff0c\u7ec6\u80de\u5916\u90e8\u7535\u6d41\u573a\u4ea7\u751f\u7684\u7ec6\u80de\u4e24\u6781\u7535\u538b\u90fd\u88ab\u7ec6\u80de\u819c\u627f\u53d7\uff0c\u7ec6\u80de\u8d28\u5185\u5206\u5230\u7684\u7535\u538b\u53ef\u4ee5\u5ffd\u7565\u4e0d\u8ba1\uff0c\u7ec6\u80de\u8d28\u5185\u90e8\u51e0\u4e4e\u6ca1\u6709\u7535\u6d41\uff0c\u56e0\u6b64\u4e5f\u51b3\u5b9a\u4e86\u6b63\u5e38\u8303\u56f4\u5185\u7684\u7535\u8f6c\u8fc7\u7a0b\u4e2d\u7ec6\u80de\u6bd2\u6027\u5f88\u5c0f\u3002\u7535\u573a\u4f7fDNA\u7b49\u7269\u8d28\u8fdb\u5165\u7ec6\u80de\u819c\u540e\u53ea\u80fd\u505c\u6b62\u5728\u7ec6\u80de\u819c\u9644\u8fd1\uff0c\u968f\u540e\u7ec6\u80de\u672c\u8eab\u7684\u673a\u5236\u53ef\u4ee5\u5141\u8bb8\u8fd9\u4e9b\u7269\u8d28\u5230\u7ec6\u80de\u6838\u7b49\u5904\u3002 \u666e\u901a\u7535\u8f6c\u80fd\u91cf\u8fc7\u9ad8\u53ef\u5bfc\u81f4\u90e8\u5206\u7ec6\u80de\u56e0\u819c\u7834\u574f\u800c\u6b7b\u4ea1\uff0c\u5176\u4e2d\u80fd\u5b58\u6d3b\u4e0b\u6765\u7684\u7ec6\u80de\u5185\u90e8\u90fd\u4e0d\u4f1a\u53d7\u5230\u7535\u6d41\u5f71\u54cd\uff0c\u56e0\u4e3a\u7ec6\u80de\u819c\u5b8c\u6574\u662f\u7ec6\u80de\u5b58\u6d3b\u7684\u5fc5\u8981\u6761\u4ef6\u3002 \u7531\u4e8e\u7535\u8f6c\u6280\u672f\u4f9d\u9760\u7684\u662f\u7269\u7406\u65b9\u6cd5\uff0c\u7ec6\u80de\u8868\u9762\u7684\u5206\u5b50\u7279\u6027\u5bf9\u7535\u8f6c\u5f71\u54cd\u6bd4\u8f83\u5c0f\u3002\u76f8\u6bd4\u5316\u5b66\u8f6c\u67d3\u65b9\u6cd5\u548c\u75c5\u6bd2\u8f7d\u4f53\u8f6c\u67d3\u65b9\u6cd5\uff0c\u7535\u8f6c\u53ef\u4ee5\u7528\u5728\u6240\u6709\u7684\u7ec6\u80de\u79cd\u7c7b\u4e0a\uff0c\u800c\u4e14\u5bb9\u6613\u5b9a\u91cf\u63a7\u5236\u3002 2. \u7ec6\u80de\u7535\u8f6c\u7535\u573a\u56fe \u00a0\u7ec6\u80de\u819c\u8fd1\u4f3c\u7edd\u7f18\u4f53\uff0c\u7ec6\u80de\u9644\u8fd1\u7535\u8f6c\u6db2\u4e2d\u7535\u6d41\u626d\u66f2\u3002 \u00a0\u5728\u4e32\u8054\u7535\u8def\u4e2d\u7535\u963b\u8d8a\u5927\u5206\u5230\u7535\u538b\u8d8a\u5927\uff0c\u7ec6\u80de\u4e24\u7aef\u7535\u538b\u57fa\u672c\u90fd\u843d\u5728\u4e24\u7aef\u7ec6\u80de\u819c\u4e0a\u3002 \u00a0\u53ea\u6709\u4e00\u4e2a\u7ec6\u80de\u7aef\u9762\u7535\u8f6c\u6709\u6548\u3002 \u00a0\u7ec6\u80de\u8d28\u5185\u5206\u5230\u7535\u538b\u5f88\u5c0f\uff0cDNA\u7535\u8f6c\u8fdb\u5165\u7ec6\u80de\u819c\u540e\u9a6c\u4e0a\u505c\u6b62\uff0c\u540e\u7eed\u9760\u7ec6\u80de\u672c\u8eab\u673a\u5236\u6162\u901f\u6269\u6563\u3002 \u00a0\u7ec6\u80de\u6838\u4e24\u7aef\u7535\u538b\u53ca\u5176\u5fae\u5c0f\uff0c\u5fae\u5c0f\u7684\u7535\u538b\u53c8\u843d\u5728\u6838\u819c\u4e0a\uff0c\u6838\u5185\u90e8\u5b8c\u5168\u6ca1\u6709\u7535\u538b\uff0c\u7535\u8f6c\u6ca1\u6709\u57fa\u56e0\u6bd2\u6027\u3002 3. \u7535\u8f6c\u6280\u672f\u5e94\u7528 \u6709\u6548\u7684\u7ec6\u80de\uff1a\u51e0\u4e4e\u6240\u6709\u7684\u771f\u6838\u7ec6\u80de\uff0c\u5305\u62ec\u6240\u6709\u7ec6\u80de\u7cfb\uff0c\u539f\u4ee3\u7ec6\u80de\uff0c\u5e72\u7ec6\u80de\uff0c\u8840\u6db2\u548c\u514d\u75ab\u7c7b\u7ec6\u80de\uff0c\u795e\u7ecf\u7ec6\u80de\uff0c\u690d\u7269\u7ec6\u80de\u548c\u80da\u80ce\u3002 \u53ef\u4ee5\u7535\u8f6c\u7684\u7269\u8d28\u5305\u62ecDNA\u3001RNA\u3001siRNA\u3001\u86cb\u767d\u8d28\u7b49\u3002 \u9002\u7528\u5b9e\u9a8c\uff1a\u77ac\u65f6\u6216\u7a33\u5b9a\u8868\u8fbe\u86cb\u767d\u8d28\u3001\u57fa\u56e0\u6572\u9664\u4e0e\u6572\u5165\u3001siRNA\u548c\u5176\u4ed6\u5206\u5b50\u673a\u5236\u7814\u7a76\u3002 \u79d1\u7814\u9886\u57df\uff1a\u7ec6\u80de\u5206\u5b50\u751f\u7269\u5b66\u3001\u514d\u75ab\u5b66\u3001\u8840\u6db2\u5b66\u3001\u795e\u7ecf\u5b66\u3001\u764c\u75c7\u7814\u7a76\u3001\u65b0\u836f\u5f00\u53d1\u7b49\u3002 4. \u4e3a\u4ec0\u4e48\u7535\u8f6c\u76f4\u63a5\u8fdb\u5165\u7ec6\u80de\u6838\u662f\u9519\u8bef\u7684 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