{"id":39,"date":"2021-10-28T08:15:51","date_gmt":"2021-10-28T08:15:51","guid":{"rendered":"http:\/\/www.celetrix.cn\/celetrix\/?page_id=39"},"modified":"2023-08-02T06:14:32","modified_gmt":"2023-08-02T06:14:32","slug":"nucleus-transfection","status":"publish","type":"page","link":"https:\/\/www.celetrix.cn\/zh\/nucleus-transfection\/","title":{"rendered":"\u8f6c\u67d3\u7ec6\u80de\u6838\u9519\u8bef\u6982\u5ff5"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"39\" class=\"elementor elementor-39\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-28463444 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"28463444\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-4bdc6109\" data-id=\"4bdc6109\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3ea4b548 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"3ea4b548\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.cn\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>\u5168\u65b0\u9ad8\u6548\u7ec6\u80de\u7535\u8f6c\u4eea<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2b5aaa55 elementor-widget elementor-widget-heading\" data-id=\"2b5aaa55\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u4e3a\u4ec0\u4e48\u7535\u8f6c\u76f4\u63a5\u8fdb\u5165\u7ec6\u80de\u6838\u662f\u9519\u8bef\u7684<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9f1454e elementor-widget elementor-widget-text-editor\" data-id=\"9f1454e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div>1.\u7ec6\u80de\u6838\u8f6c\u67d3\u8fdd\u53cd\u7269\u7406\u5b66\u539f\u7406\u3002<\/div><p>\u7ec6\u80de\u819c\u8fd1\u4f3c\u7edd\u7f18\u4f53\uff0c\u7ec6\u80de\u9644\u8fd1\u7535\u8f6c\u6db2\u4e2d\u7535\u6d41\u626d\u66f2\u3002\u5728\u4e32\u8054\u7535\u8def\u4e2d\u7535\u963b\u8d8a\u5927\u5206\u5230\u7535\u538b\u8d8a\u5927\uff0c\u7ec6\u80de\u4e24\u7aef\u7535\u538b\u57fa\u672c\u90fd\u843d\u5728\u4e24\u7aef\u7ec6\u80de\u819c\u4e0a\u3002\u53ea\u6709\u4e00\u4e2a\u7ec6\u80de\u7aef\u9762\u7535\u8f6c\u6709\u6548\u3002\u7ec6\u80de\u8d28\u5185\u5206\u5230\u7535\u538b\u5f88\u5c0f\uff0cDNA\u7535\u8f6c\u8fdb\u5165\u7ec6\u80de\u819c\u540e\u9a6c\u4e0a\u505c\u6b62\uff0c\u540e\u7eed\u9760\u7ec6\u80de\u672c\u8eab\u673a\u5236\u6162\u901f\u6269\u6563\u3002\u7ec6\u80de\u6838\u4e24\u7aef\u7535\u538b\u53ca\u5176\u5fae\u5c0f\uff0c\u5fae\u5c0f\u7684\u7535\u538b\u53c8\u843d\u5728\u6838\u819c\u4e0a\uff0c\u6838\u5185\u90e8\u5b8c\u5168\u6ca1\u6709\u7535\u538b\uff0c\u7535\u8f6c\u6ca1\u6709\u57fa\u56e0\u6bd2\u6027\u3002<\/p><p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-full wp-image-525\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095628_133.png\"  width=\"536\" height=\"272\" srcset=\"https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095628_133.png 536w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095628_133-300x152.png 300w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095628_133-18x9.png 18w\" sizes=\"(max-width: 536px) 100vw, 536px\" title=\"20210610095628_133.png\" alt=\"20210610095628_133.png\" \/><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-19e860cb elementor-widget elementor-widget-text-editor\" data-id=\"19e860cb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>2.\u6587\u732e\u4e2d\u5df2\u7ecf\u62a5\u5bfc\u7535\u8f6c\u540eDNA\u4ec5\u5728\u7ec6\u80de\u819c\u9644\u8fd1\uff08Golzio et al.\uff09\u3002<\/p><p><img decoding=\"async\" class=\"alignnone size-full wp-image-526\" src=\"http:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095647_972.png\"  width=\"503\" height=\"431\" srcset=\"https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095647_972.png 503w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095647_972-300x257.png 300w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210610095647_972-14x12.png 14w\" sizes=\"(max-width: 503px) 100vw, 503px\" title=\"20210610095647_972.png\" alt=\"20210610095647_972.png\" \/><\/p><p>3.\u7ec6\u80de\u6838\u8f6c\u67d3\u6ca1\u6709\u5b9e\u9a8c\u8bc1\u636e\u3002<\/p><p>4.\u7ec6\u80de\u672c\u8eab\u53ef\u4ee5\u8ba9DNA\u8fdb\u5165\u7ec6\u80de\u6838\u3002\u6bd4\u5982Celetrix\u7535\u8f6c\u540e30\u5206\u949f\u5c31\u53ef\u4ee5\u770b\u5230GFP\u8367\u5149\u8868\u8fbe\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-592139fb\" data-id=\"592139fb\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4ab7601d elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"4ab7601d\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas 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menu-item-object-page menu-item-98\"><a href=\"https:\/\/www.celetrix.cn\/zh\/celetrix-advantage\/\" class=\"elementor-item\">Celetrix\u7ec6\u80de\u7535\u8f6c\u4f18\u52bf<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-103\"><a href=\"https:\/\/www.celetrix.cn\/zh\/vs-cuvette\/\" class=\"elementor-item\">\u4e0e\u7535\u6781\u676f\u6bd4\u8f83<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-102\"><a href=\"https:\/\/www.celetrix.cn\/zh\/vs-capillary\/\" class=\"elementor-item\">\u4e0e\u6bdb\u7ec6\u7ba1\u6bd4\u8f83<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-101\"><a href=\"https:\/\/www.celetrix.cn\/zh\/rnp-mrna-sirna-vs-plasmid\/\" class=\"elementor-item\">mRNA\/ siRNA\u7535\u8f6c<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-4ab7601d\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-99\"><a href=\"https:\/\/www.celetrix.cn\/zh\/electroporation-mechanism\/\" class=\"elementor-item\" tabindex=\"-1\">\u7535\u8f6c\u57fa\u672c\u539f\u7406<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-100\"><a href=\"https:\/\/www.celetrix.cn\/zh\/nucleus-transfection\/\" class=\"elementor-item\" tabindex=\"-1\">\u8f6c\u67d3\u7ec6\u80de\u6838\u9519\u8bef\u6982\u5ff5<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-98\"><a href=\"https:\/\/www.celetrix.cn\/zh\/celetrix-advantage\/\" class=\"elementor-item\" tabindex=\"-1\">Celetrix\u7ec6\u80de\u7535\u8f6c\u4f18\u52bf<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-103\"><a href=\"https:\/\/www.celetrix.cn\/zh\/vs-cuvette\/\" class=\"elementor-item\" tabindex=\"-1\">\u4e0e\u7535\u6781\u676f\u6bd4\u8f83<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-102\"><a href=\"https:\/\/www.celetrix.cn\/zh\/vs-capillary\/\" class=\"elementor-item\" tabindex=\"-1\">\u4e0e\u6bdb\u7ec6\u7ba1\u6bd4\u8f83<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-101\"><a href=\"https:\/\/www.celetrix.cn\/zh\/rnp-mrna-sirna-vs-plasmid\/\" class=\"elementor-item\" tabindex=\"-1\">mRNA\/ siRNA\u7535\u8f6c<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page \u4e3a\u4ec0\u4e48\u7535\u8f6c\u76f4\u63a5\u8fdb\u5165\u7ec6\u80de\u6838\u662f\u9519\u8bef\u7684 1.\u7ec6\u80de\u6838\u8f6c\u67d3\u8fdd\u53cd\u7269\u7406\u5b66\u539f\u7406\u3002 \u7ec6\u80de\u819c\u8fd1\u4f3c\u7edd\u7f18\u4f53\uff0c\u7ec6\u80de\u9644\u8fd1\u7535\u8f6c\u6db2\u4e2d\u7535\u6d41\u626d\u66f2\u3002\u5728\u4e32\u8054\u7535\u8def\u4e2d\u7535\u963b\u8d8a\u5927\u5206\u5230\u7535\u538b\u8d8a\u5927\uff0c\u7ec6\u80de\u4e24\u7aef\u7535\u538b\u57fa\u672c\u90fd\u843d\u5728\u4e24\u7aef\u7ec6\u80de\u819c\u4e0a\u3002\u53ea\u6709\u4e00\u4e2a\u7ec6\u80de\u7aef\u9762\u7535\u8f6c\u6709\u6548\u3002\u7ec6\u80de\u8d28\u5185\u5206\u5230\u7535\u538b\u5f88\u5c0f\uff0cDNA\u7535\u8f6c\u8fdb\u5165\u7ec6\u80de\u819c\u540e\u9a6c\u4e0a\u505c\u6b62\uff0c\u540e\u7eed\u9760\u7ec6\u80de\u672c\u8eab\u673a\u5236\u6162\u901f\u6269\u6563\u3002\u7ec6\u80de\u6838\u4e24\u7aef\u7535\u538b\u53ca\u5176\u5fae\u5c0f\uff0c\u5fae\u5c0f\u7684\u7535\u538b\u53c8\u843d\u5728\u6838\u819c\u4e0a\uff0c\u6838\u5185\u90e8\u5b8c\u5168\u6ca1\u6709\u7535\u538b\uff0c\u7535\u8f6c\u6ca1\u6709\u57fa\u56e0\u6bd2\u6027\u3002 2.\u6587\u732e\u4e2d\u5df2\u7ecf\u62a5\u5bfc\u7535\u8f6c\u540eDNA\u4ec5\u5728\u7ec6\u80de\u819c\u9644\u8fd1\uff08Golzio et al.\uff09\u3002 3.\u7ec6\u80de\u6838\u8f6c\u67d3\u6ca1\u6709\u5b9e\u9a8c\u8bc1\u636e\u3002 4.\u7ec6\u80de\u672c\u8eab\u53ef\u4ee5\u8ba9DNA\u8fdb\u5165\u7ec6\u80de\u6838\u3002\u6bd4\u5982Celetrix\u7535\u8f6c\u540e30\u5206\u949f\u5c31\u53ef\u4ee5\u770b\u5230GFP\u8367\u5149\u8868\u8fbe\u3002<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"class_list":["post-39","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.celetrix.cn\/zh\/wp-json\/wp\/v2\/pages\/39","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.cn\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.cn\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.cn\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.cn\/zh\/wp-json\/wp\/v2\/comments?post=39"}],"version-history":[{"count":8,"href":"https:\/\/www.celetrix.cn\/zh\/wp-json\/wp\/v2\/pages\/39\/revisions"}],"predecessor-version":[{"id":3456,"href":"https:\/\/www.celetrix.cn\/zh\/wp-json\/wp\/v2\/pages\/39\/revisions\/3456"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.cn\/zh\/wp-json\/wp\/v2\/media?parent=39"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}