{"id":57,"date":"2021-10-28T08:17:46","date_gmt":"2021-10-28T08:17:46","guid":{"rendered":"http:\/\/www.celetrix.cn\/celetrix\/?page_id=57"},"modified":"2023-08-02T06:43:50","modified_gmt":"2023-08-02T06:43:50","slug":"special-cells","status":"publish","type":"page","link":"https:\/\/www.celetrix.cn\/zh\/special-cells\/","title":{"rendered":"\u7ec6\u80de\u7535\u8f6c\u670d\u52a1"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"57\" class=\"elementor elementor-57\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-34033061 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"34033061\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-2d31dfb\" data-id=\"2d31dfb\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5e598527 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"5e598527\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.cn\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>\u5168\u65b0\u9ad8\u6548\u7ec6\u80de\u7535\u8f6c\u4eea<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7db203b1 elementor-widget elementor-widget-text-editor\" data-id=\"7db203b1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><strong>\u7ec6\u80de\u7535\u8f6c<\/strong><\/h3><p>Celetrix\u7535\u8f6c\u51e0\u4e4e\u4e0d\u6311\u7ec6\u80de\u79cd\u7c7b\uff0c\u90fd\u53ef\u4ee5\u8fbe\u5230\u9ad8\u6548\u7387\u3002\u6709\u4e00\u90e8\u5206\u7ec6\u80de\u79cd\u7c7b\uff0c\u7531\u4e8e\u5176\u672c\u8eab\u7279\u6709\u7684\u6027\u8d28\uff0c\u5728\u7535\u8f6c\u65f6\u9700\u8981\u7279\u522b\u6ce8\u610f\u91c7\u7528\u5408\u9002\u7684\u65b9\u6848\u3002<\/p><p>\u5355\u6838\u7ec6\u80de\u548c\u5de8\u566c\u7ec6\u80de\u76f8\u5173\u7684\u7ec6\u80de\u7cfb\uff0c\u6709\u4e9b\u7535\u8f6c\u8d28\u7c92\u540e\u7ec6\u80de\u4f1a\u5728\u4e24\u4e09\u5929\u5185\u51e0\u4e4e\u5168\u90e8\u6b7b\u4ea1\u3002THP-1\uff0cU937\uff0cHL-60\u548cMolm13\u7b49\u7ec6\u80de\u7535\u8f6c\u8d28\u7c92\u540e\u4f1a\u6b7b\u4ea1\u3002\u5176\u4ed6\u8001\u5f0f\u7684\u7535\u8f6c\u4eea\u7535\u8f6c\u8d28\u7c92\u5230\u8fd9\u4e9b\u7ec6\u80de\u540e\u4f1a\u6709\u90e8\u5206\u7ec6\u80de\u5b58\u6d3b\uff0c\u662f\u56e0\u4e3a\u5176\u4e2d\u6709\u5f88\u591a\u6ca1\u6709\u7535\u8f6c\u6210\u529f\u7684\u7ec6\u80de\u6d3b\u4e0b\u6765\u4e86\uff0c\u5bf9\u4e8e\u7535\u8f6c\u6ca1\u6709\u4ec0\u4e48\u610f\u4e49\u3002<\/p><p>Celetrix\u4e13\u95e8\u5f00\u53d1\u4e86\u8bd5\u5242\u76d2\u53ef\u4ee5\u5e2e\u52a9\u5355\u6838\u7ec6\u80de\u7c7b\u7684\u7ec6\u80de\u7cfb\u8fdb\u884c\u8d28\u7c92\u7535\u8f6c\uff0c\u76ee\u524d\u9a8c\u8bc1\u8fc7THP-1\u548cU937\u7ec6\u80de\u8f6c\u5165\u8d28\u7c92\u540e\u90e8\u5206\u7ec6\u80de\u53ef\u4ee5\u5b58\u6d3b\u751f\u957f\uff0c\u4ece\u800c\u4f7f\u96be\u505a\u7684\u7ec6\u80de\u682a\u7a33\u8f6c\u53d8\u5f97\u5f88\u7b80\u5355\u3002\u4e0b\u56fe\u6240\u793aTHP-1\u7ec6\u80de\u7535\u8f6cCas9-gRNA-Puro-GFP\u8d28\u7c92\u65f6\u4ec5\u75281x10E6\u7ec6\u80de\u5c31\u53ef\u4ee5\u7b5b\u9009\u5f97\u5230\u7a33\u8f6c\u514b\u9686\u5e76\u4e14\u6210\u529f\u8fdb\u884c\u57fa\u56e0\u7f16\u8f91\u3002<\/p><div><img fetchpriority=\"high\" decoding=\"async\" class=\"size-full wp-image-230 aligncenter\" src=\"http:\/\/celetrix.hy-yun.com\/wp-content\/uploads\/2021\/11\/20210719112316_816.png\"  width=\"698\" height=\"328\" srcset=\"https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210719112316_816.png 698w, https:\/\/www.celetrix.cn\/wp-content\/uploads\/2021\/11\/20210719112316_816-300x141.png 300w\" sizes=\"(max-width: 698px) 100vw, 698px\" title=\"20210719112316_816.png\" alt=\"20210719112316_816.png\" \/><\/div><div><p>THP-1\u8fd9\u4e9b\u7ec6\u80de\u7535\u8f6cmRNA,\u00a0siRNA\u548c\u00a0Cas9 RNP\u6ca1\u6709\u7ec6\u80de\u6b7b\u4ea1\u7684\u95ee\u9898\u3002\u5982\u679c\u53ef\u4ee5\u4e0d\u4f7f\u7528\u8d28\u7c92\uff0c\u7535\u8f6c\u975e\u5e38\u5bb9\u6613\uff0c\u6548\u7387\u63a5\u8fd1100%\u5e76\u4e14\u7ec6\u80de\u5b58\u6d3b\u975e\u5e38\u597d\u3002<\/p><p>\u6211\u4eec\u7684THP-1\u8bd5\u5242\u76d2\u5bf9\u4e8eMolm13\u548cSUP-M2\u7ec6\u80de\u7684\u8d28\u7c92\u7535\u8f6c\u5e2e\u52a9\u4e0d\u5927\uff0c\u6211\u4eec\u5728\u7ee7\u7eed\u7814\u7a76\u5176\u8d28\u7c92\u7535\u8f6c\u65b9\u6cd5\u3002<\/p><p>\u5c0f\u9f20\u7684\u539f\u4ee3\u9aa8\u9ad3\u5206\u79bb\u7684\u5355\u6838\u7ec6\u80de\u548c\u9020\u8840\u5e72\u7ec6\u80de\u975e\u5e38\u7279\u6b8a\uff0c\u4e0d\u4ec5\u7535\u8f6c\u8d28\u7c92\u540e\u5bb9\u6613\u6b7b\u4ea1\uff0c\u4e5f\u4f1a\u8fc5\u901f\u964d\u89e3mRNA\u548csiRNA\u9020\u6210\u7535\u8f6c\u4e0d\u6210\u529f\u7684\u5047\u8c61\u3002\u53ea\u8981\u7535\u8f6c\u6db2\u548cmRNA\u548c\u8fd9\u4e9b\u7ec6\u80de\u63a5\u89e61\u5206\u949f\uff0c\u518d\u8f6c\u79fb\u5230\u5176\u4ed6\u7ec6\u80de\u4e0a\u7535\u8f6c\u5c31\u4f1a\u53d1\u73b0mRNA\u5df2\u7ecf\u5b8c\u5168\u964d\u89e3\u3002\u5c0f\u9f20\u813e\u810f\u7ec6\u80de\u4e5f\u6709\u964d\u89e3mRNA\u548csiRNA\u7684\u7279\u6027\u3002\u800c\u5c0f\u9f20\u8179\u8154\u7684\u5de8\u566c\u7ec6\u80de\u867d\u7136\u7535\u8f6c\u8d28\u7c92\u540e\u5bb9\u6613\u6b7b\u4ea1\uff0c\u7535\u8f6cmRNA\u5374\u6ca1\u6709\u964d\u89e3\u95ee\u9898\u53ef\u4ee5\u4f7f\u5b9e\u9a8c\u987a\u5229\u8fdb\u884c\u3002<\/p><p>\u7535\u8f6c\u5b9e\u9a8c\u4e2d\u78b0\u5230\u7279\u6b8a\u73b0\u8c61\u65f6\uff0c\u7528\u6237\u53ef\u4ee5\u53d1\u90ae\u4ef6\u5411\u6211\u4eec\u54a8\u8be2\uff0c\u6211\u4eec\u53ef\u4ee5\u63d0\u4f9b\u5176\u4ed6\u5b9e\u9a8c\u65b9\u6848\u4f9b\u9009\u62e9\u3002<\/p><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-706df8f8\" data-id=\"706df8f8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-234570d elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"234570d\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot; aria-hidden=\\&quot;true\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-234570d\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.cn\/zh\/manuals-videos\/\" class=\"elementor-item\">\u4eea\u5668\u64cd\u4f5c\u89c6\u9891\u4e0e\u8d44\u6599\u4e0b\u8f7d<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.cn\/zh\/recommend-parameters\/\" class=\"elementor-item\">\u5e38\u89c1\u7ec6\u80de\u7535\u8f6c\u53c2\u6570\u63a8\u8350<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.cn\/zh\/optimization-strategy\/\" class=\"elementor-item\">\u7ec6\u80de\u7535\u8f6c\u5e38\u89c1\u95ee\u9898<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.cn\/zh\/experimental-overview\/\" class=\"elementor-item\">\u5e38\u7528\u7ec6\u80de\u7535\u8f6c\u7ed3\u679c<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.cn\/zh\/special-cells\/\" class=\"elementor-item\">\u7ec6\u80de\u7535\u8f6c\u670d\u52a1<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-234570d\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.cn\/zh\/manuals-videos\/\" class=\"elementor-item\" tabindex=\"-1\">\u4eea\u5668\u64cd\u4f5c\u89c6\u9891\u4e0e\u8d44\u6599\u4e0b\u8f7d<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.cn\/zh\/recommend-parameters\/\" class=\"elementor-item\" tabindex=\"-1\">\u5e38\u89c1\u7ec6\u80de\u7535\u8f6c\u53c2\u6570\u63a8\u8350<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.cn\/zh\/optimization-strategy\/\" class=\"elementor-item\" tabindex=\"-1\">\u7ec6\u80de\u7535\u8f6c\u5e38\u89c1\u95ee\u9898<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.cn\/zh\/experimental-overview\/\" class=\"elementor-item\" tabindex=\"-1\">\u5e38\u7528\u7ec6\u80de\u7535\u8f6c\u7ed3\u679c<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.cn\/zh\/special-cells\/\" class=\"elementor-item\" tabindex=\"-1\">\u7ec6\u80de\u7535\u8f6c\u670d\u52a1<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>\u5168\u65b0\u9ad8\u6548\u7ec6\u80de\u7535\u8f6c\u4eea \u9875\u9762 \u7ec6\u80de\u7535\u8f6c Celetrix\u7535\u8f6c\u51e0\u4e4e\u4e0d\u6311\u7ec6\u80de\u79cd\u7c7b\uff0c\u90fd\u53ef\u4ee5\u8fbe\u5230\u9ad8\u6548\u7387\u3002\u6709\u4e00\u90e8\u5206\u7ec6\u80de\u79cd\u7c7b\uff0c\u7531\u4e8e\u5176\u672c\u8eab\u7279\u6709\u7684\u6027\u8d28\uff0c\u5728\u7535\u8f6c\u65f6\u9700\u8981\u7279\u522b\u6ce8\u610f\u91c7\u7528\u5408\u9002\u7684\u65b9\u6848\u3002 \u5355\u6838\u7ec6\u80de\u548c\u5de8\u566c\u7ec6\u80de\u76f8\u5173\u7684\u7ec6\u80de\u7cfb\uff0c\u6709\u4e9b\u7535\u8f6c\u8d28\u7c92\u540e\u7ec6\u80de\u4f1a\u5728\u4e24\u4e09\u5929\u5185\u51e0\u4e4e\u5168\u90e8\u6b7b\u4ea1\u3002THP-1\uff0cU937\uff0cHL-60\u548cMolm13\u7b49\u7ec6\u80de\u7535\u8f6c\u8d28\u7c92\u540e\u4f1a\u6b7b\u4ea1\u3002\u5176\u4ed6\u8001\u5f0f\u7684\u7535\u8f6c\u4eea\u7535\u8f6c\u8d28\u7c92\u5230\u8fd9\u4e9b\u7ec6\u80de\u540e\u4f1a\u6709\u90e8\u5206\u7ec6\u80de\u5b58\u6d3b\uff0c\u662f\u56e0\u4e3a\u5176\u4e2d\u6709\u5f88\u591a\u6ca1\u6709\u7535\u8f6c\u6210\u529f\u7684\u7ec6\u80de\u6d3b\u4e0b\u6765\u4e86\uff0c\u5bf9\u4e8e\u7535\u8f6c\u6ca1\u6709\u4ec0\u4e48\u610f\u4e49\u3002 Celetrix\u4e13\u95e8\u5f00\u53d1\u4e86\u8bd5\u5242\u76d2\u53ef\u4ee5\u5e2e\u52a9\u5355\u6838\u7ec6\u80de\u7c7b\u7684\u7ec6\u80de\u7cfb\u8fdb\u884c\u8d28\u7c92\u7535\u8f6c\uff0c\u76ee\u524d\u9a8c\u8bc1\u8fc7THP-1\u548cU937\u7ec6\u80de\u8f6c\u5165\u8d28\u7c92\u540e\u90e8\u5206\u7ec6\u80de\u53ef\u4ee5\u5b58\u6d3b\u751f\u957f\uff0c\u4ece\u800c\u4f7f\u96be\u505a\u7684\u7ec6\u80de\u682a\u7a33\u8f6c\u53d8\u5f97\u5f88\u7b80\u5355\u3002\u4e0b\u56fe\u6240\u793aTHP-1\u7ec6\u80de\u7535\u8f6cCas9-gRNA-Puro-GFP\u8d28\u7c92\u65f6\u4ec5\u75281x10E6\u7ec6\u80de\u5c31\u53ef\u4ee5\u7b5b\u9009\u5f97\u5230\u7a33\u8f6c\u514b\u9686\u5e76\u4e14\u6210\u529f\u8fdb\u884c\u57fa\u56e0\u7f16\u8f91\u3002 THP-1\u8fd9\u4e9b\u7ec6\u80de\u7535\u8f6cmRNA,\u00a0siRNA\u548c\u00a0Cas9 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