Celetrix Electroporation

全新高效细胞电转仪
不同规格电击管电压换算

 不同电击管的电压参数需要换算,时间相同。以20ul电击管参数为基准,100ul20ul约上调10V200ul20ul的约2.4倍;1000ul20ul2.5倍;120ul的是20ul的约1.4倍,600ul的是20ul的约1.6倍。下面是转换示例:

20ul500V

100ul: 510V  20ul电压+10V

200ul1200V 20ul电压的2.4倍)

1000ul1250V 20ul电压的2.5倍)

120ul700V 20ul电压的1.4倍)

600ul800V 20ul电压的1.6倍)

*部分单核细胞相关的细胞系,电转质粒后几乎所有电转成功的细胞会死亡,而电转mRNA,siRNA和Cas9 RNP没关系。Celetrix公司专门开发了试剂盒可以帮助单核细胞类的细胞系进行质粒电转,目前验证过THP-1和U937这两种细胞系电转效果非常好,而Molm-13和Sup-M2细胞电转质粒细胞死亡问题还没有解决。

Cell Type  Mode 20ul, 100ul  200ul, 1ml 
Human PBMC PBMC 1300 -1450 NA
Human T cells, unstimulated PBMC 1300 -1450 NA
Human B cells, unstimulated PBMC 1300 -1450 NA
Human NK cells, unstimulated PBMC 1300 -1450 NA
Human Neutrophil PBMC 1300 -1450 NA
Mouse lymph node primary T cell PBMC 1300 -1450 NA
Mouse primary spleen cells PBMC 1300 -1450 NA
Mouse bone marrow monocyte PBMC 1300 -1450 NA
Human Cultured T cells Cell Line 480-540 1150-1300
Human Cultured NK cells Cell Line 500-540 1200-1300
Human iPSC Cell Line 500-540 1200-1300
Human MM1.S Cell Line 520-560 1250-1350
Human adipose MSC Cell Line 500-540 1200-1300
Human bone marrow MSC Cell Line 500-540 1200-1300
Human cord blood MSC Cell Line 500-540 1200-1300
Human ESC derived neural stem cell Cell Line 520-560 1250-1350
Human primary hepatocyte Cell Line 480-520 1150-1250
Human primary Dendritic cells  Cell Line 480-500 1150-1200
Human neural progenitor ReNCell VM Cell Line 520-560 1250-1350
Mouse iPSC Cell Line 480-520 1150-1250
Mouse ESC Cell Line 480-520 1150-1250
Mouse lymph node Cultured T cell Cell Line 500-540 1200-1300
Mouse peritoneal Macrophage Cell Line 560-580 1350-1400
Primary mouse cortical neurons Cell Line 560-580 1350-1400
Rat primary cortex neural progenitor cell Cell Line 520-560 1250-1350
293 Cell Line 440-480 1050-1150
293T Cell Line 440-480 1050-1150
A549 Cell Line 520-560 1250-1350
ADSCs Cell Line 500-540 1200-1300
ARPE-19 Cell Line 520-560 1250-1350
BJ Cell Line 520-560 1250-1400
CHO-K1 Cell Line 520-580 1250-1400
CHO-S Cell Line 520-580 1250-1400
DEL Cell Line 500-540 1200-1300
Hela Cell Line 520-560 1250-1350
Hep3b Cell Line 460-500 1100-1200
HSF Cell Line 520-560 1250-1350
HUC-Maaa13-P3 Cell Line 520-560 1250-1350
HUVEC Cell Line 520-560 1250-1350
H1975 Cell Line 520-560 1250-1350
H226 Cell Line 520-560 1250-1350
H9C2 Cell Line 560-580 1350-1400
HL-60 * Cell Line 520-560 1250-1350
iMC Cell Line 500-540 1200-1300
Jurkat Cell Line 500-560 1200-1350
Karpas299 Cell Line 520-560 1250-1350
K562 Cell Line 480-520 1200-1300
MDA-MB-231 Cell Line 500-540 1220-1300
Molm-13 * Cell Line 520-560 1250-1350
Nalm-6 Cell Line 520-560 1250-1350
NK-92 Cell Line 480-500 1150-1200
Raji Cell Line 520-560 1250-1350
RAW264.7 Cell Line 520-560 1250-1350
SF9 insect cell Cell Line 480-520 1200-1300
SK-HEP-1 Cell Line 500-540 1220-1300
SP2/0 Cell Line 480-500 1150-1200
SUP-M2 * Cell Line 520-560 1250-1350
Swiss-3T3 Cell Line 520-560 1250-1350
p3X63Ag8.653 Cell Line 500-540 1220-1300
THP-1 * Cell Line 520-540 1250-1300
U251 Cell Line 520-560 1250-1400
U937 * Cell Line 520-540 1250-1300

*Many monocyte or macrophage related cell lines such as THP-1, U937, HL-60 and Molm13 will die out within two to three days after plasmid electroporation. These cells have no problem with mRNA, siRNA and Cas9 RNP electroporation. The efficiency would be nearly 100% and cell survival is not affected. Celetrix has developed the THP-1 Helper Kit for plasmid electroporation of monocytic cell lines. A significant portion of THP-1 and U937 cells can survive and grow with the helper kit, enabling easy generation of stable cell lines. Our THP-1 Helper Kit does not help cell lines such as Molm13 and SUP-M2 with plasmid electroporation and we are actively researching the solutions.